The Granulose Reaction of Certain Anaerobes of the "Butyric" Group.

نویسنده

  • R S Spray
چکیده

McCoy, Fred, Peterson, and Hastings (1926) state: "One of the most distinctive features of the group to which the butyl organism belongs is the granulose reaction. Young vegetative cells.. . stain yellow with iodine. As sporulation approaches and the cells take on clostridial form, they store granulose and stain blue or violet with iodine. As the spores mature the granulose reaction is lost, indicating that the reserve material has been utilized in sporulation." This microscopic observation has long been used as a criterion for the classification of members of this iodophilic group. Batchelor and Curie (1929) have grown cultures of soil butyrics in oat jars, then flooded the plates with iodine, and determined the iodophiles by the macroscopic observation of the blue-black colonies. In reporting a study of the cultivation and identification of the butyric anaerobes (Spray, 1937), there was discussed a striking phenomenon observed in semisolid agar cultures of certain members of this group. This reaction was obtained when Gram's iodine was added to glucose semisolid agar cultures, which immediately turned dark violet. This reaction was consistently obtained with only certain members of the group and thus appears to have a possible value for the differentiation of certain species. In view of the chaotic taxonomy of the butyric group, such a "species" character may prove of the same significance as the "stormy fermentation" of Clostridium perfringens, the "iron-gelatin" reaction of Clostridium histolyticum, and the "vanillin-violet" (skatol) reaction of Clostridium sporogenes and Clostridium parabotulinum (Spray, 1936). The conditions under which granulose is formed have been a matter of considerable study. We mention here only the study of Svartz (1930), who states, "The typical iodophil, clostridium-forming bacteria in the feces never deposited iodophil substance in a medium with a pH of less than about 6.6, even when there was plenty of carbohydrate in the medium." This held true also for a series of butyrics from various sources. We have confirmed this statement in regard to a variety of butyrics obtained through the courtesy of Dr. Elizabeth McCoy and Dr. Ivan Hall-including McCoy's Clostridium saccharobutyricum (Wis. 63), Clostridium felsineum (Wis. 41), Clostridium pasteurianum (Wis. 60), Granulobacter saccharobutyriusimmobile-non-liquefaciens (Wis. 24), Clostridium butyricum-iodophilum (Wis. 61), Clostridium butylicus (Wis. 39), "unknown butyric" (Wis. 33), Clostridium acetobutylicum (Wis.), Clostridium roseum (Wis. 43), and a series of "unknown butyrics" from Dr. Hall, nos. 9039, 9041, 3234, 7241, 3813, 274, 9040, 9042, and 9043. However, these facts did not seem to apply to certain strains, including

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عنوان ژورنال:
  • Journal of bacteriology

دوره 55 1  شماره 

صفحات  -

تاریخ انتشار 1948